High Sensitivity Analysis of Diarrhetic Shellfish Poisoning (DSP) Toxins Using Liquid Chromatography Tandem Mass Spectrometry

For the past decades marine toxins in shellfish have been monitored by the mouse bioassay (MBA) in many countries including Japan. Recently several alternative testing methods have been developed and a few of them have been validated. The most widely accepted method for many kinds of the marine toxins is liquid chromatography (LC) combined with mass spectrometry (MS), deemed to be the powerful tool than the MBA in sensitivity and accuracy. Diarrhetic shellfish poisoning (DSP) toxins, okadaic acid (OA) and dinophysistoxins (DTXs), are very important target for marine bio-toxin monitoring in Japan. The MBA for DSP toxin monitoring was replaced with a LC/MS/MS method on the new regulation issued in March 2015 in Japan (Notification No. 1 issued by the Dept. of Food Safety, Pharmaceutical and Food Safety Bureau, Japanese Ministry of Health, Labour and Welfare on March 6, 2015).
In this presentation, we demonstrate the developed LC/MS/MS methods for the screening of OA, dinophysistoxin1 (DTX1), pectenotoxin1, 2, 6 (PTX) and yessotoxin (YTX) as well as for the routinely quantification of OA and DTX1.

Content Type:
Paper
Document Number:
PO-CON1524E
Product Type:
Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
shellfish, Food and Beverages, Food safety (Residues, Contaminants), LCMS-8050
Language:
English
File Name:
jpo115043.pdf
File Size:
940kb

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