Integration of amino acid, acylcarnitine and steroids analysis in single FIA/LC-MS/MS platform

Analysis of amino acids (AA) and acylcarnitines (AC) in dried blood spot (DBS) sample collection method by flow injection analysis (FIA) is now widely used. On the other hand, traditionally, analysis of steroid such as 17-hydroxyprogesterone is done by immunoassays but LC/MS/MS will be an attractive analytical alternative because it can also screen for other related steroids. The use of LC/MS/MS results in a reduction of false positives and a more accurate quantitative performance. The requirements against steroid analysis by LC/MS/MS are getting more stringent issues. In this study, we present a strategy for performing both AA/AC and steroids analysis within a single LC/MS/MS platform.