Quantification and structural characterization of glycans and glycopeptides by TQMS: the energy-resolved oxonium ion monitoring (Erexim) platform

We have previously reported the method of using a triple quadrupole mass spectrometer to reproducibly monitor glycan-derived low molecular weight ions (oxonium ions) to acquire, within milliseconds, a profile of fragmentation patterns at a range of CID collision energies. This, which we termed the energy-resolved oxonium ion monitoring (Erexim) profile, reflects the glycan structure in such a way that isobaric structures can be clearly distinguished and assigned. Here we have developed new software to fully support Erexim data acquisition and analysis. Moreover, we acquired the Erexim profile database using purified glycan standards to enable glycan structure identification by pattern matching. The Erexim platform thus gives solution to both structural characterization and quantitative analysis of glycan and glycopeptides.

Content Type:
Paper
Document Number:
PO-CON1558E
Product Type:
Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
glycans, glycopeptides, oxonium ion, Pharmaceutical, Life Science, LCMS-8050
Language:
English
File Name:
jpo115072.pdf
File Size:
958kb

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