Rice bran contains about 18 to 20 % oil, which is used as rice oil. A key characteristic of rice oil is its stability with respect to oxidation, due to its low content of linolenic acid, which is a polyunsaturated fatty acid. The rice germ portion of rice bran, which is known for its high nutritional value, contains high levels of rice oil. However, extracting it requires using hexane or other organic solvents and is time-consuming.
Therefore, we evaluated the use of the DART (Direct Analysis in Real Time) method to analyze the lipids by simply exposing the rice bran to direct analysis, without involving solvent extraction or any other pretreatment process.