Quantitative Analysis of Steroid Hormones Using Triple Quadrupole LC/MS/MS

Steroid hormones are heavily involved with the control of metabolism, neurotransmission, and intracellular signaling, playing critical roles in the proper functioning of the body. Further, not only do steroids play roles in sedation and seizure prevention, they are known to be effective in cancer treatment and regenerative medicine. Steroid quantitation in biological samples is therefore an important tool in clinical research.
This Application News introduces the analysis of steroid hormones in human serum by LC/MS/MS following pretreatment using ISOLUTE^® SLE+ (supported liquid extraction).