Optimization and control of cell culture processes are essential to increase production efficiency of biopharmaceuticals. In the field of cell therapy, enhanced control of the culture process is also becoming important to reduce cell variability and improve consistency of mass production of the cells. Comprehensive monitoring of culture supernatant components gives researchers useful information for these purposes. However, current technologies for process monitoring are limited to measurement of pH, dissolving gases, and some small compounds such as glucose, glutamine, lactate, and ammonia. We have developed a “Cell Culture Media Analysis Platform, C2MAP system” that can perform automated sample pretreatment and simultaneous analysis of up to 95 compounds listed below. We also developed a viewer software that can easily visualize temporal change in each measured compounds through the cell culture. In this poster, we present features of C2MAP system and its applications.