A rapid screening method of mycotoxins in grains by liquid chromatograph tandem mass spectrometry

Mycotoxin is a chemical substance produced by mold. In terms of harmful substances to health of human and livestock, regulatory limitation is defined in each country. In recent years, the risk management of mycotoxins has been gaining wider acceptance all over the world. In this report, we demonstrate a simultaneous screening analysis for 18 kinds of mycotoxins in grains by LC/MS/MS.
For the purification of grain extract, Multitoxin Spin Column (Romer Labs) was utilized in order to perform a simple and rapid clean-up treatment for mycotoxins, which has individual chemical properties, such as aflatoxins, ochratoxin A, trichothecenes, and fumonisins.