High-Sensitivity Analysis of a Steroid Panel Samples using Micro-Flow LC-MS/MS for Clinical Research

As they are involved in many physiological processes, steroids are important biomarkers to assess health status and to investigate environmental effects on exposed populations. In pediatric or post-menopausal samples, circulating levels of reproductive hormones are very low and therefore challenging to measure. In addition, sample volume availability can be limited adding difficulties for high-sensitivity analysis.
Here we present a method to simultaneously measure seventeen steroids in serum samples using micro-flow LC/MS/MS at high sensitivity.