In recent years, new synthetic drugs have appeared in the illicit market. These compounds include various analogs and newly emerged compounds with different chemical structures. One of the major points of concern is the substantial ineffectiveness of current toxicological sample pretreatment and screening methods to identify the compounds in biological samples. The recent increase in the sales
and abuse of substituted drugs highlighted the need for fast and reliable identification of such compounds with a simple sample preparation technology everybody can easily and smoothly implement. This work describes the identification workflow of pre-spiked drugs treated with micro-volume QuEChERS extraction protocol using high resolution accurate mass spectrometry.