MRM based phospholipid profiling of mouse tissues by an ultra-fast triple quadrupole mass spectrometer

Phospholipids (PLs) serve as cell membrane structure holding various fatty acids such as arachidonic acid (AA) or docosahexaenoic acid (DHA). Although multiple reaction monitoring (MRM) using triple quadrupole mass spectrometer (TQ-MS) is expected to be the better sensitivity than scan or Q-TOF based method, theoretical number of PLs reaches several thousands, which is hard to be covered by MRM based method. Hence we have narrowed down the target PLs to develop over 400 polar head monitoring MRM and over 800 MRM for fatty acid determination using an ultra-fast TQ-MS. The MRM based method is expected to be better for finding out the minor PLs. Here we report PLs profiling results of mouse tissues using the MRM based method.