Overcoming Challenges of Protein Sample Preparation for Food Allergen Analysis
An on-line sample preparation workstation that automates proteolysis, de-salting and reversed phase chromatography for proteomic workflows has been described.Two major benefits of the system as they relate to food analysis are reproducbility and speed. By configuring the system to enable multiplexing, we have successfully shown the ability to digest and analyze 200 samples per day.This sample preparation method has been applied to analyze Ara h 1, a common food allergen associated with peanuts. Complete digestion of the Ara h 1 protein was demonstrated reproducibly, with identification of the common tryptic peptidesfound in Ara h 1 protein. One common peptide, SFNLDEGHALR (m/z 629.8) was identified by MS/MS with abundant representation of b and y ions.
- Content Type:
- Paper
- Document Number:
- PO-CON1362E
- Product Type:
- Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
- Keywords:
- Peanuts, Peptide, Food Allergen, Food and Beverages, Food QAQC, Food safety (Residues, Contaminants), LCMS-IT-TOF
- Language:
- English
- File Name:
- sio113076.pdf
- File Size:
- 655kb