Online affinity and digestion: A flexible and robust tool for the characterization and quantification of proteins

Traditional, manual proteolytic digest and immuno-affinity sample preparation techniques have been plagued by their lack of qualitative and quantitative reproducibility. This has contributed in part to the lack of implementation of protein biomarkers in quantitative MS-based assays. Online automation of digestion can reduce variability, user error, and improve quantitative results. If coupled with online affinity capture using immobilized antibodies or affinity columns, a unique front-to-end solution can be utilized that allows reproducible sample analysis without time-consuming manual protocols. This work demonstrates the flexibility and utility of a completely online, automated solution for LC-MS/MS sample preparation and peptide analysis using both data dependent and targeted workflows.

Content Type:
Paper
Document Number:
PO-CON1486E
Product Type:
Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
proteins, Pharmaceutical, Life Science, Proteomics, LCMS-8050, LCMS IT-TOF
Language:
English
File Name:
sio114085.pdf
File Size:
1,548kb

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