There has been growing concern about flea collar pesticide effects on animal and human health. Tetrachlorvinphos (TCVP) is an organophosphate insecticide that works by affecting the central nervous system. TCVP is an EPA listed possible carcinogen still used in dog collars. Other collars in the US use a combination of pesticides, such as flumethrin and imidacloprid. On-line supercritical fluid extraction - supercritical chromatographymass spectrometry (SFE-SFC-MS) is a new analytical technique for the extraction, separation, and detection of compounds in a single analysis that limits the need for extensive manual sample preparation. The extracted compounds are trapped directly onto an analytical column for chromatographic analysis.
This technique was applied to the analysis of the above pesticides in store bought dog collars.