Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay

The use of natural and synthetic hormones to increase the weight of meat producing animals is prohibited in the European Union (European Council Directive 96/22/EC and amended by Directive 2003/74/EC) to protect consumers from the harmful effects of digesting hormone residues and their metabolites. Compliance with this legislation is monitored by the screening of a large number of animals every year. Urine is collected from animals on farms and liver from animals at abattoirs. Typically, sample preparation of these urine samples involves time-consuming two-step offline-SPE. Here, we have developed an online-SPE method to considerably shorten the pre-treatment time. In addition to optimisation of the extraction method, chromatographic separation was optimised to decrease ion-suppression and isobaric interference. Compound ionisation was enhanced using a new heated ESI (HESI) source on the LCMS-8050 triple quadrupole mass spectrometer.

Content Type:
Paper
Document Number:
PO-CON1484E
Product Type:
Liquid Chromatography, Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
urine, Food and Beverages, Food safety (Residues, Contaminants), LCMS-8050, Nexera UHPLC
Language:
English
File Name:
uko114076.pdf
File Size:
1,086kb

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