Study of experimental conditions for MALDI Imaging by using highly controlled sublimation technique
Sublimation technique for coating matrices is known to generate highly uniform and fine crystals of matrices, which enables to visualize precise distribution of targeted molecule in MALDI imaging. A thickness of coated matrix is necessary to be controlled to achieve high reproducibility. However, controlling the thickness is frequently hard, because it is managed only by elapsed time of sublimation. Measuring increased weight of the coated subject is reliable, but it should be done after coating. Recently, we developed a new sublimation technique, in which the thickness of matrix is monitored in real time by variation of laser light transmission. We will report an experimental condition of the sublimation technique for MALDI imaging and apply it to a new vacuum MALDI-TOF/TOF.
- Content Type:
- Paper
- Document Number:
- PO-CON1535E
- Product Type:
- Mass Spectrometry, Life Science Lab Instruments, MALDI-TOF Mass Spectrometry
- Keywords:
- coated matrix, Hydrocarbon Processing Industry (Petrochemical, Chemical), MALDI-7090, MALDI-TOF-MS, MALDI-TOF-TOF
- Language:
- English
- File Name:
- jpo315049.pdf
- File Size:
- 3,739kb