Serotype-Level Bacterial Discrimination Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

We successfully achieved serotype-level discrimination of enterohemorrhagic Escherichia coli using a proteotyping method (the S10-GERMS method) with markers consisting of ribosomal proteins detected during bacterial measurements using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). In the future, this technique could be utilized as a routine method for the discrimination of bacteria at levels finer than the species level, which has thus far not been possible with conventional MALDI-TOF MS fingerprinting-based microbial identification methods.

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Life Science Lab Instruments, Mass Spectrometry, MALDI-TOF Mass Spectrometry
food-poisoning pathogens, MALDI-TOF MS, proteotyping, S10-GERMS, Strain Solution Ver. 2, Food, Beverages, Pharmaceutical, Life Science, Clinical research, Forensics, AXIMA
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