Simple Evaluation of Cultured Cells: Evaluation of Differentiation State of iPS Cells Using Benchtop MALDI-TOF MS

The growth condition and differentiation state (differentiated or undifferentiated) of cultured cells are important evaluation items in regenerative medicine and basic research. If these cell evaluations can be conducted in a simple manner by mass spectrometry, evaluation time and complicated work can be significantly reduced.
This article reports the results of an automatic analysis of iPS cells and their culture medium by Aura Solution using the Shimadzu MALDI-8020 benchtop linear MALDI-TOF mass spectrometer and a trial preliminary analysis of the evaluation of the cultured cell state by the statistical analysis software eMSTAT Solution.