Detection of Multi-Base Mutation by Genome Editing Using MultiNA™

In genome editing, it is necessary to ascertain whether editing is on-target or not. Although heteroduplex mobility assay (HMA) is a simple method for this purpose, confirmation of editing is impossible in some cases due to differences in multiple bases (from 1 to 5 base pairs). In cases where detection is not possible by HMA, the enzyme mismatch cleavage method using the enzymes called T7 endonuclease I (T7EI) and Cel 1 is effective.
This article introduces an example of analysis in which quick and simple detection of mismatch cleavage by T7EI was possible by using a Shimadzu microchip electrophoresis system.

Content Type:
Document Number:
Product Type:
Life Science Lab Instruments, Microchip Electrophoresis
Genome editing, TALEN, CRISPR /Cas9, knockout, experimental animal, selective breeding, genome, proteome, peptide, protein, cell, ES cell, iPS cell, artificial nuclease, Pharmaceutical, Life Science, MCE-202, MultiNA
File Name:
File Size:

View Article

For Research Use Only. Not for use in diagnostic procedures.

This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.

Top of This Page