Detection of Multi-Base Mutation by Genome Editing Using MultiNA™

In genome editing, it is necessary to ascertain whether editing is on-target or not. Although heteroduplex mobility assay (HMA) is a simple method for this purpose, confirmation of editing is impossible in some cases due to differences in multiple bases (from 1 to 5 base pairs). In cases where detection is not possible by HMA, the enzyme mismatch cleavage method using the enzymes called T7 endonuclease I (T7EI) and Cel 1 is effective.
This article introduces an example of analysis in which quick and simple detection of mismatch cleavage by T7EI was possible by using a Shimadzu microchip electrophoresis system.

Content Type:
Application
Document Number:
B110
Product Type:
Life Science Lab Instruments, Microchip Electrophoresis
Keywords:
Genome editing, TALEN, CRISPR /Cas9, knockout, experimental animal, selective breeding, genome, proteome, peptide, protein, cell, ES cell, iPS cell, artificial nuclease, Pharmaceutical, Life Science, MCE-202, MultiNA
Language:
English
File Name:
jpu220002.pdf
File Size:
180kb

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For Research Use Only. Not for use in diagnostic procedures.

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