Amino Acid Sequence Analysis of Peptides and Proteins with Modified Amino Acid Using PPSQ™-50A Isocratic System

The expressed proteins undergo a variety of post-translational modifications in living cells and become mature proteins with the function specific to those proteins. The proteins in databases are registered as precursor proteins, and therefore, protein analysis using a mass spectrometer may give a low score for search results. Amino acids that have been modified after translation can be identified easily by using a protein sequencer employing Edman degradation as a complementary analytical device.
This article introduces an example of amino acid sequence analysis of a synthetic peptide with a modified amino acid by using the PPSQ-50A protein isocratic system.