Structural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry

MS2/MS3 was able to characterise with high detail the N-glycans of ovarian cancer cells and able to identify the different isobaric N-glycans occupying a single MS1 peak. Furthermore, with controlled high-energy fragmentation, MS3/MS4 was able to confirm and characterise antenna modifications such as sialylation and fucosylation based on diagnostic ions.
In order to enhance the peak signals, the spotting procedure was also optimised by comparing the matrices DHB (2,5-dihydroxybenzoic acid) and DABP (3,4-diaminobenzophenone).
Although DHB and DAPB produced similar results, the ionisation process of DHB was found to be more efficient in lower mass range i.e. 1500-2500 Da. Therefore it was chosen as the matrix for MS3 and MS4 experiments.
Further experiments using the hybrid high vacuum MALDI-QIT-TOF instruments will consist of using this method for characterisation of a larger set of glycans relying in the high sensitivity and MSn capabilities of the instrument.

Content Type:
Paper
Document Number:
PO-CON1347E
Product Type:
Mass Spectrometry, Life Science Lab Instruments, MALDI-TOF Mass Spectrometry
Keywords:
N-glycan, Ovarian Cancer, Glycosylation, Pharmaceutical, Life Science, Metabolomics, Development (Formulation, Scale-up, Method development), AXIMA Resonance
Language:
English
File Name:
kro313052.pdf
File Size:
326kb

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