Three-Dimensional Spectra Measurement of Fluorescent Probes used for DNA Detection
DNA probes labeled with fluorescent dye (below referred to as fluorescent probes) are used extensively to detect and identify specific DNA when conducting life science studies. The mechanism involves the selective binding of the probe to specific DNA, thereby permitting the detection of that DNA. However, due to the wide variety of fluorescent dyes, it is important to know the exact wavelength at which the probe fluoresces to ensure DNA detection. Here, using the three-dimensional spectral measurement feature of the RF-6000 Spectrofluorophotometer, we introduce examples of fluorescence measurement of two types of fluorescent probes.
- Content Type:
- Application
- Document Number:
- LAAN-A-UV-E047
- Product Type:
- Molecular Spectroscopy, Fluorescence Spectroscopy
- Keywords:
- DNA, Fluorescent probe, Fluorescent dye, Fluorescent labeling, Fluorescence measurement, Pharmaceutical, Life Science, RF-6000
- Language:
- English
- File Name:
- jpa415011.pdf
- File Size:
- 1,184kb