Fluorescence Spectrum Measurement of Fluorescence Dye Indocyanine Green in Long Wavelength Region

In the medical and biochemical fields, the use of fluorescent dye for labeling specific molecules is a commonly used technique for the observation of tissue lesions. Indocyanine Green fluorescent dye displays fluorescence in the near infrared region in the vicinity of 810 nm. Measurement in wavelength regions greater than 750 nm has been difficult using conventional spectrofluorophotometers, but the newly-developed Shimadzu RF-6000 spectrofluorophotometer permits measurement over a wide wavelength range from 200 to 900 nm. Furthermore, the automatic spectral correction feature of this instrument makes it possible to automatically obtain comparable data from different instruments.
Here, we introduce an example of measurement of Indocyanine Green using the RF-6000.