Low-Concentration Measurement of Duloxetine Hydrochloride by RF-6000 Spectrofluorophotometer

The sensitivity of fluorescence spectroscopy is 100 to 1,000 times higher than that of absorption photometry because light emission intensity is detected from the zero state. As a result, fluorescence spectroscopy is suitable for measurement of low-concentration samples. This technique is particularly well-suited for use in the development of pharmaceutical products with limited sample amounts, and for research in the life science field involving biological samples. Preparations of digitoxin and digoxin, which are cardiac glycosides of digitalis, are quantified in the Japanese Pharmacopoeia.
Here, the antidepressant duloxetine hydrochloride was measured, and its lower limit of detection and lower limit of quantitation were evaluated.