Micro Volume Photometric Measurement of Nucleic Acid using TrayCell with UV-1280

Nucleic acid concentration can be determined easily using an ultraviolet-visible (UV-VIS) spectrophotometer. Such samples are typically available in small micro volume (less than 5 ?l), and a system that is able to measure micro volume with high reproducibility will be preferred. A conventional 10 mm path length super micro cuvette requires at least 50 ?l of sample and needs extra care in cleaning to prevent carryover. Hellma TrayCell is a fiber-optic ultra-micro cell designed for the UV-VIS micro volume analysis of nucleic acids and proteins. The sample volume requirement ranges from 0.7 ?l to 10 ?l depending on the path length used. Shimadzu UV-1280 offers a bio method mode as a standard function for DNA and protein concentrations determination. This application news demonstrates the photometric measurement of micro-volume of nucleic acid with good repeatability using UV-1280 together with Hellma TrayCell.