Nucleic acid concentration can be determined easily using an ultraviolet-visible (UV-VIS) spectrophotometer. Such samples are typically available in small micro volume (less than 5 ?l), and a system that is able to measure micro volume with high reproducibility will be preferred. A conventional 10 mm path length super micro cuvette requires at least 50 ?l of sample and needs extra care in cleaning to prevent carryover. SINCO Nano Stick microliter cuvette is designed for the UV-VIS micro volume analysis of nucleic acids and proteins. The minimum sample volume required is 2 ?l. This application news demonstrates the quantitation measurement of micro-volume of nucleic acid with good repeatability using UV-1280 together with Nano Stick.