Trace Quantity Measurement System
This trace quantity measurement system can perform measurements simply and accurately, even with ultra-small sample volumes on the order of a few μL. It is optimal for DNA and protein analyses in the life sciences.
Capable of Measurements with Just a Droplet of a Sample
To perform the measurement, drip a droplet of the sample and then cap it. After measurement, just wipe away the sample, and proceed with the next analysis.
Effective for the Measurement of Thick, High-Concentration Samples
Dilution Not Required
As the optical path length is shorter than the 10 mm cell, 10x to 50x concentration samples can be measured directly.
The interior of an ultra micro cell is narrow. As a result, the cleaning process is difficult and contamination is a problem. With the trace quantity cell, after measurement, just wipe the window clean with a cotton swab.
Difficult for Bubbles to Enter
Placing a sample in an ultra micro cell without bubbles entering is difficult. With the trace quantity cell, however, the sample is just dripped, making it difficult for bubbles to enter.
Nano Stick Example
Select the Instrument and Cell to Suit the Intended Use
The combination of a UV-VIS spectrophotometer and the trace quantity measurement cell can be selected to suit the intended use.
Use the double beam UV-1800 or single beam UV-1280 spectrophotometer. Two types of trace quantity measurement cells are available.
SCINCO Nano Stick-S™
With a 0.5 mm optical path length, this cell requires a mere 2 μL of a sample. Measurements can be performed simply, just by dripping one droplet of the sample. After the measurement, just wipe clean the sampling port.
With an optical path length of 0.2 mm or 1 mm, this cell requires 0.7 to 5 μL of a sample. The optical path length can be changed by changing the cap.
Measurements can be performed simply, just by dripping one droplet of the sample onto the TrayCell™ window and then capping it. After the measurement, just wipe clean the window and the inside of the cap.
Quantitation of Trace Quantities of Nucleic Acids
Samples of nucleic acids and proteins are often only available in small quantities. Accordingly, cells capable of trace quantity measurements are required. In this example, trace quantity measurements of nucleic acids were performed using a TrayCell™ with an optical path length of 1.0 mm, which allows measurements with sample volumes of 3 to 5 μL.
Lambda-DNA, a type of double stranded DNA often utilized in nucleic acid analysis, was analyzed.
A 1.0 Abs concentration sample was measured repeatedly 10 times with good precision, as follows:
The standard deviation was 0.014629 with a CV of 1.34 %.
The calibration curve formula was Y = 0.00201x + 0.00827, and the correlation coefficient was r2 = 0.99989.
Overlaid Spectra for Nucleic Acids with Different Concentrations