Efficient Method Development of Small Interfering RNA by Reversed-Phase Ion-Pair Chromatography

Due to the effort of the RNAinterfering(RNAi)to prevent protein synthesis, short-duplexes of RNA, such as small interfering RNA(siRNA)is being studied as a treatment for a variety of indications[1]. The methods for characterization of oligonucleotide therapeutics are required from development phase to quality control of aproduct.Ion-PairReversed-Phase Liquid Chromatography(IP-RPLC) under denaturing conditions is typically used for siRNA characterization. Separation from sense and antisense strands and contaminants is important for more accurate results but achieving an accurate analytical method requires consideration of complex parameters such as column chemistry,ion-pair reagent concentration, and column temperature. This study describes how to achieve the optimal separation on siRNA and related-impurities efficiently by LabSolutions MD which is a dedicated software for supporting method development.