Ampdirect Technology works without RNA purification

PCR inhibition appears to be caused by positively-charged substances in the biological sample (some types of protein etc.) which are adsorbed to the DNA/RNA template, and some negatively-charged substances (some sugars, pigments etc.) which are adsorbed to the DNA polymerase. Ampdirect has the effect of suppressing these substances, enabling direct RT-PCR of biological samples.

Ampdirect Technology works without RNA purification

Literature review

For Research Use Only. Not for use in diagnostic procedures.


Genotyping of single nucleotide polymorphism (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip
Hiratsuka M, et al.: Drug Matab. Pharmacokin. 2004;19: 303-7

Rapid and simple mutation screening of GM1 gangliosidosis in Shiba dogs by direct amplification of deoxyribonucleic acid from various forms of canine whole-blood specimens
Yamato O, et al.: J Vet Diagn Invest 2004;16: 469-72

Prenatal origin of TEL-AMLI-positive acute lymphoblastic leukemia in children born in California
McHale CM, et al GENES, Chromosomes & Cancer 2003;37: 36-43

In utero origin of t(8;21) AML1-ETO translocations in childhood acute myeloid leukemia
Wiemels JL, et al.: Blood 2002;99: 3801-5

Site-specific translocation and evidence of postnatal origin of the t(1;19) E2A-PBX1 fusion in childhood acute lymphoblastic leukemia
Wiemels JL, et al.: Proc. Natl. Acad. Sci. USA 2002;99: 15101-6

Various applications of direct PCR using blood samples
Nishimura N, et al.: Clin Lab; 2002;48: 377-84

Efficiency of polymerase chain reaction using a novel method of DNA preparation and amplification for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples
Kojima K, et al.: Proceedings of the Seventh International Colloquium on Paratuberculosis; 2002; 267-9

Major change in the predominant type of "Norwalk-like viruses" in outbreaks of acute nonbacterial gastroenteritis in Osaka city, Japan, between April 1996 and March 1999
Iritani, et al.: J Clin Microbiol 2000;38: 2649-54

Detection of clonotypic IGH and TCR rearrangements in the neonatal blood spots of infants and children with B-cell precursor acute lymphoblastic leukemia
Yagi T, et al.: Blood; 2000;96:264-8

Direct PCR from whole blood without DNA isolation
Nishimura N, et al.: Ann Clin Biochem; 2000;37: 674-80

Association between platelet glycoprotein Iba genotype and ischemic cerebrovascular disease
Sonoda A, et al.: Stroke 2000;31: 493-7

Prenatal origin of acute lymphoblastic leukaemia in children
Wiemels JL, et al.: Lancet 1999;354: 1499-503

Direct amplification of Escherichia coli O157 vero toxin genes from human faeces by the polymerase chain reaction
Okamoto H, et al.: Ann Clin Biochem 1999;36: 642-8

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