Ampdirect™ - Features
Ampdirect Technology works without RNA purification
PCR inhibition appears to be caused by positively-charged substances in the biological sample (some types of protein etc.) which are adsorbed to the DNA/RNA template, and some negatively-charged substances (some sugars, pigments etc.) which are adsorbed to the DNA polymerase. Ampdirect has the effect of suppressing these substances, enabling direct RT-PCR of biological samples.
Literature review
For Research Use Only. Not for use in diagnostic procedures.
Genotyping of single nucleotide polymorphism (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip
Hiratsuka M, et al.: Drug Matab. Pharmacokin. 2004;19: 303-7
Rapid and simple mutation screening of GM1 gangliosidosis in Shiba dogs by direct amplification of deoxyribonucleic acid from various forms of canine whole-blood specimens
Yamato O, et al.: J Vet Diagn Invest 2004;16: 469-72
Prenatal origin of TEL-AMLI-positive acute lymphoblastic leukemia in children born in California
McHale CM, et al GENES, Chromosomes & Cancer 2003;37: 36-43
In utero origin of t(8;21) AML1-ETO translocations in childhood acute myeloid leukemia
Wiemels JL, et al.: Blood 2002;99: 3801-5
Site-specific translocation and evidence of postnatal origin of the t(1;19) E2A-PBX1 fusion in childhood acute lymphoblastic leukemia
Wiemels JL, et al.: Proc. Natl. Acad. Sci. USA 2002;99: 15101-6
Various applications of direct PCR using blood samples
Nishimura N, et al.: Clin Lab; 2002;48: 377-84
Efficiency of polymerase chain reaction using a novel method of DNA preparation and amplification for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples
Kojima K, et al.: Proceedings of the Seventh International Colloquium on Paratuberculosis; 2002; 267-9
Major change in the predominant type of "Norwalk-like viruses" in outbreaks of acute nonbacterial gastroenteritis in Osaka city, Japan, between April 1996 and March 1999
Iritani, et al.: J Clin Microbiol 2000;38: 2649-54
Detection of clonotypic IGH and TCR rearrangements in the neonatal blood spots of infants and children with B-cell precursor acute lymphoblastic leukemia
Yagi T, et al.: Blood; 2000;96:264-8
Direct PCR from whole blood without DNA isolation
Nishimura N, et al.: Ann Clin Biochem; 2000;37: 674-80
Association between platelet glycoprotein Iba genotype and ischemic cerebrovascular disease
Sonoda A, et al.: Stroke 2000;31: 493-7
Prenatal origin of acute lymphoblastic leukaemia in children
Wiemels JL, et al.: Lancet 1999;354: 1499-503
Direct amplification of Escherichia coli O157 vero toxin genes from human faeces by the polymerase chain reaction
Okamoto H, et al.: Ann Clin Biochem 1999;36: 642-8
Ampdirect is a trademark of Shimadzu Corporation