Application
News
No. G320
The Nexis GC-2030 gas chromatograph has recently
begun to support packed columns. The detectors
compatible with packed columns are a flame
ionization detector (FID) and a thermal conductivity
detector (TCD).
In this article, the amount of ethanol in liquor was
analyzed using the Nexis GC-2030 (FID) with a glass-
packed column in accordance with the analytical
method prescribed by the Japanese National Tax
Agency.
C. Kanamori, K. Kawamoto
Packed Column Support System
The glass column that can be installed into the Nexis GC-
2030, a system compatible with packed columns, is the
same as that used for the GC-17 or GC-2010, and it can
be shared between these instruments.
Using the FID-2030Packed kit (P/N: S221-85191-41), the
FID-2030 can be easily modified to become a packed FID
by:
• Replacing the standard nozzle for the FID with one for a
packed FID;
• Removing the FID’s capillary column adaptor.
Fig. 1 shows the appearance of the instrument and an
example of glass column installation.
Example of Analysis
Analysis of Ethanol in Liquor
According to the analytical method prescribed by the
Japanese National Tax Agency, ethanol, as the
standard sample, was diluted with water and prepared
at concentrations of 5%, 10%, 15% and 20% (v/v). To
0.1 mL of each ethanol standard solution, 0.9 mL of the
internal standard solution (acetone) was added to
make samples that were analyzed to create the
calibration curve. Actual samples of beer and Japanese
sake available on the market were prepared. In the
same manner as above, 0.9 mL of the internal standard
solution was added to 0.1 mL of each liquor sample.
These samples were analyzed, and the ethanol was
quantified using the calibration curve created.
Analysis Conditions
Table 1 lists the configuration of the instrument used
for analysis and the analysis conditions.
Table 1 Instrument Configuration and Analysis Conditions
Gas Chromatography
Packed Column Analysis of Ethanol in Liquor
Using Nexis™ GC-2030 (FID)
Fig. 1 Appearance of Nexis™ GC-2030 and Example of Glass
Column Installation
Model : Nexis GC-2030 /AOC-20i
+SINJ-2030+FID-2030Packed Kit
Injection Mode : Direct
Injection Volume : 1.0 μL
Injection Temp. : 200 °C
Carrier Gas :N
2
Carrier Gas Control : Constant flow rate (35 mL/min)
Column : PEG 1000 10% CW 60/80 AW-DMCS
(2 m × 3 mm I.D.)
Column Temp. : 100 °C
Detector : Flame ionization detector (FID)
Detector Temp. : 200 °C
Detector Gas :H
2
32.0 mL/min,
Air 200 mL/min
(no makeup gas required)
Extendable to Dual Line
A packed column analysis of samples with a column
temperature program may cause greater baseline drift
due to the elution of the liquid phase from the column,
which can affect identification or quantitative
processing.
The FID-2030 (compatible with packed columns) can be
extended to a dual line system comprising a reference
line, which has a sample vaporization chamber, column
and detector, for elimination of baseline drift, as well as
the sample line for analyzing samples.
