Workshops : Osaka University Shimadzu Omics Innovation Research Laboratories : SHIMADZU CORPORATION

We are offering workshops on metabolomics and other topics using pretreatment equipment and mass spectrometers in the Analytical Innovation Research Laboratory.
Participants in the workshops gain hands-on experience in the procedures for various research by pretreating and measuring samples and then analyzing the data measured by mass spectrometers.

Note: A workshop participation fee will be charged. The workshop schedule may be subject to change.

Workshop Schedule
Food Metabolomics Workshop (intended for the food industry)
Workshop Number and Location
The 3rd	Food Metabolomics Workshop	February 8 (Wed) and 9 (Thu), 2017, in Osaka	Time: 9:30 A.M. to 5:30 P.M. (Day 1), 9:30 A.M. to 4:00 P.M. (Day 2) Fee: 48,000 yen (consumption tax included) Maximum participants: 6 (minimum 4 participants required for the workshop)
The 2nd	Mass Imaging Pretreatment Workshop	November 1 (Tue) and 2 (Wed), 2016, in Osaka	Time: 1:00 P.M. to 5:40 P.M. (Day 1), 10:00 A.M. to 3:30 P.M. (Day 2) Fee: 100,000 yen (consumption tax included) Maximum participants: 10
The 1st	Food Metabolomics Workshop	December 17 (Thu) and 18 (Fri), 2015, in Osaka	Time: 9:30 A.M. to 5:30 P.M. (Day 1), 9:30 A.M. to 4:00 P.M. (Day 2) Fee: 48,000 yen (consumption tax included) Maximum participants: 6 (minimum 3 participants required for the workshop)

＜The 3rd Food Metabolomics Workshop Program＞

Contents

Participants in the workshop will pretreat food samples for analysis, perform measurements using the GC-MS/MS and LC-MS/MS, and then analyze the measured data (including multivariate analyses). Through this workshop, participants will gain hands-on experience in the procedures for analyzing metabolomics, and learn differences in using GC-MS/MS and LC-MS/MS.

Intended for

This workshop is intended for people who are starting food metabolomics analysis using GC-MS(/MS) and LC-MS/MS. People who do not have access to the instruments can also participate in this workshop.

Instruments Used

GCMS-TQ8040
(Smart Metabolites Database)
LCMS-8060
(LC/MS/MS Method Package for Primary Metabolites Ver. 2)

Day 1	AM	・Orientation ・Sample pretreatment Participants will pretreat and prepare samples to be measured. ・Preparing instruments for measurement Participants will adjust the instruments (perform tuning).
Day 1	PM	・Sample measurement Participants will measure the pretreated samples using mass spectrometers. ・Presentation on food metabolomics and discussion A presentation on food metabolomics will be given by Professor Eiichiro Fukusaki, Osaka University.
Day 2	AM	・Presentation on imaging metabolomics and description of the demonstration A presentation on imaging metabolomics will be given by Associate Professor Shuuichi Shimma, Osaka University. ・Data analysis (Mass spectrometer) Participants will analyze the data measured on Day 1.
Day 2	PM	・Data analysis (Multivariate analysis) ・Q&A

＜The 2nd Mass Imaging Pretreatment Workshop Program＞

Contents

There will be hands-on work and discussions regarding both the appropriate freezing methods and slicing procedures as well as the matrix application methods that are extremely important when performing mass imaging on biological samples.

Intended for

This workshop is intended for people who are already performing mass imaging using iMScope.

Instruments Used

iMScope
iMLayer
LEICA CM1950

Day 1	PM	・Orientation ・Classroom lecture on sample section preparation ・Practical session on freezing and slicing ・Practical session on vapor deposition ・Classroom lecture on the 2-step matrix application method ・Discussion（Q&A）・Measurement using iMScope
Day 2	AM	・Practical session on matrix spraying ・Measurement using iMScope ・Description of the SFE/SFC system
Day 2	PM	・Introduction to post staining technique after sample measurement ・Checking measurement results ・Discussion（Q&A）

＜The 1st Food Metabolomics Workshop Program＞

Contents

Intended for

This workshop is intended for people who are starting food metabolomics analysis using GC-MS(/MS) and LC-MS/MS. People who do not have access to the instruments can also participate in this workshop.

Instruments Used

GCMS-TQ8040
(Smart Metabolites Database)
LCMS-8060
(LC/MS/MS Method Package for Primary Metabolites Ver. 2)

Day 1	AM	・Orientation ・Sample pretreatment Participants will pretreat and prepare samples to be measured. ・Preparing instruments for measurement Participants will adjust the instruments (perform tuning).
Day 1	PM	・Sample measurement Participants will measure the pretreated samples using mass spectrometers. ・Presentation on food metabolomics and discussion A presentation on food metabolomics will be given by Professor Eiichiro Fukusaki, Osaka University.
Day 2	AM	・Data analysis (Mass spectrometer) Participants will analyze the data measured on Day 1.
Day 2	PM	・Data analysis (Multivariate analysis) ・Q&A