March 7, 2016 | News & Notices New Integrated LC Makes Updating Traditional HPLC Methods to UHPLC Easy
- Nexera-i MT's dual flow lines permit UHPLC and HPLC analysis on a single system -

Nexera-i MT System

Nexera-i MT System

Shimadzu has released the new Nexera-i MT UHPLC system, which is based on the very successful i-Series platform launched in 2014, and offers enhanced functionality to support the transfer of existing HPLC methods to faster UHPLC analyses while assuring high cross-compatibility between the old and new method conditions. Nexera-i MT features two independent and dedicated flow lines, one for UHPLC and the other for HPLC. Newly developed Analytical Conditions Transfer and Optimization (ACTO) technology minimizes the effect of system volume differences on analytical results. In addition to drastically improving the efficiency and quality of method development and transfer efforts in quality control departments, Nexera-i MT's dual flow lines also maximize operational efficiency by allowing a single instrument to run both HPLC and UHPLC analyses as opposed to separate dedicated instruments for each. The innovative Nexera-i MT will be exhibited at PITTCON 2016 from March 6th to 10th in Atlanta, GA, USA.

Background to the Development

In recent years there has been a strong focus on the use of UHPLC instruments and small particle columns to develop faster and better analytical methods that improve efficiency and throughput especially in R&D environments. However, many of the analytical methods used in quality control environments, including those listed on pharmacopeia, are conventional HPLC methods. Transferring these HPLC methods to UHPLC and validating the new methods is a time consuming and labor intensive task. The Nexera-i MT incorporates two analytical flows lines with different system volumes into a single compact integrated LC. By automatically switching between the flow lines, Nexera-i MT seamlessly performs both HPLC and UHPLC analyses, preserving the relative separation pattern by automatically compensating for differences in system volume. Consequently, Nexera-i MT achieves exceptional analytical reproducibility when switching from a system with large volume to a system with small volume, or vice-versa. The same technology also allows Nexera-i MT to match any existing HPLC or UHPLC method run on competitive instrument platforms, eliminating the need to consider and carefully match plumbing to achieve identical system volumes between instruments.


1. Dual Flow Lines Permit UHPLC and HPLC Analysis on a Single System.

The Nexera-i MT features two independent analytical flow lines with different system volumes. This configuration permits UHPLC and HPLC analysis using a single system and enables the transfer of UHPLC and HPLC methods currently used in the laboratory on other instruments to Nexera-i MT without need for modification.

2. ACTO Technology Optimizes System Volume Differences.

The new ACTO technology precisely adjusts the injection timing based on the system volume difference between the existing HPLC system and Nexera-i MT, without requiring any modification to the original gradient program. According to the latest USP General Chapter <621> Chromatography, re-validation of a method is not required when injection timing is adjusted to compensate for system volume differences between instruments. The ACTO technology was developed for compliance with this USP General Chapter. Additionally, the ACTO technology can be used to automatically convert an existing HPLC method to a UHPLC method. Nexera-i MT is an ideal solution for anyone considering the conversion of existing HPLC methods to UHPLC methods, reducing mobile phase solvent consumption and enhancing productivity, and is especially attractive in environments that require for both HPLC and UHPC methods to be run on a single system.

3. Core i-Series Features Significantly Improve Analytical Efficiency.

Nexera-i MT implements the core features of the i-Series integrated LC product line including fully automated system startup, baseline stabilization, and simplified user interfaces such as the on-instrument touch-screen control panel as well as the Interactive Communication Mode (ICM), which allows the user to edit and start analysis methods and batches directly from the instrument's touch panel and automatically synchronizes with Shimadzu's LabSolutions software. The instrument occupies a small footprint and is equipped with the stable low-noise detectors and high speed, highly reproducible injectors (especially for low volume injections) that are hallmarks of Shimadzu's LC product line.

The central concept driving the development of all i-Series instruments is "To Free Operators from the Laboratory". The core features of the i-Series instruments, such as high reliability, small foot print and intuitive operation via touch-panel and smart device functionality, are highly praised by users of these instruments. The i-Series has been globally accepted in a wide range of industries including pharmaceuticals, chemicals and foods, and has been especially well-received in Europe, the Americas, Japan, India and China. The i-Series is not just an analytical instrument; its advanced design heralds the future analytical environment, the "computer-less, hassle-free laboratory."

Shimadzu plans to utilize i-Series instruments' combination of excellent performance and ease-of-use to enhance its current product offerings by developing application-specific analyzers.

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Shimadzu Nexera-i MT System