Shimadzu Review Vol.74[1・2](2017)


Analysis of 25-OH Vitamin D2/D3 and Steroids in Serum by a Fully Automated Sample Preparation LC/MS/MS System

by Daisuke Kawakami1and Toshikazu Minohata2

Shimadzu Review 74[1・2] (2017.6)


Immunoassay has been used to measure the blood concentration of drugs, steroids, and vitamin D. However, the immunoassay methods have limitations related to the differences of the methods, variation in the reagents, sensitivity, and specificity. Recently liquid chromatograph mass spectrometers (LC/MS/MS) are being used to improve analytical accuracy and precision based on its superior specificity. On the other hand, in the analysis of biological samples like serum by LC/MS/MS, sample preparation is required to carry out protein precipitation and sample dilution. These procedures cause a risk of variations and mistakes, depending on the operator’s procedure. In this article, the validation of 25-OH vitamin D2/D3 and steroids in serum using a fully automated sample preparation LC/MS/MS system is described.

Keywords: Sample preparation, Vitamin D, Steroids, LC/MS/MS

1Clinical & Biotechnology Business Unit, Life Science Business Department, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
2Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan

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