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Development of an in Vivo Real-Time Monitoring Technique by PESI/MS/MS
by Yumi Hayashi, Ph.D.1, 2, Tasuku Murata3, Kazumi Yokota4, Koretsugu Ogata, Ph.D.5, Hiroshi Tanihata4, and Kei Zaitsu, Ph.D.1, 6
(Received June 15, 2017)
Probe electrospray ionization (PESI) is an ambient ionization technique, which enables us to rapidly and directly analyze metabolites in tissue samples without tedious sample preparation. This technique uses a thin probe needle for both sampling and ionization. We have combined PESI with tandem mass spectrometry (MS/MS) to improve identification capability, and have succeeded in developing a novel analytical method to detect "intact metabolites." In this article, we introduce the application of PESI/MS/MS to directly profile the liver metabolome in acute-injury model mice. In addition, we demonstrate real-time monitoring not only for TCA cycle intermediates but also for drug metabolism in living mice.
Keywords: PESI/MS/MS, Intact metabolome analysis, Real-time metabolome analysis, Real-time monitoring
1In Vivo Real-Time Omics Laboratory, Institute for Advanced Research, Nagoya University, Nagoya, Japan
2Pathophysiological Laboratory Sciences, Department of Radiological and Medical Laboratory Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan
3Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
4Research & Development Department, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
5MS Business Unit, Life Science Business Department, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
6Department of Legal Medicine and Bioethics, Nagoya University Graduate School of Medicine, Nagoya, Japan
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