Fast Analysis of Glycoprotein-Derived Glycans Using the MultiNA
Automated Microchip Electrophoresis System
by Mitsuhiro Kinoshita, Ph.D.1, Mari Yamamoto1, Yuzu Mikoshiba1, Kazuki Matsumoto1, Sachio Yamamoto, Ph.D.1, Shigeo Suzuki, Ph.D.1, and Yuji Sogabe2
(Received December 8, 2017)
High-throughput quantitative and qualitative glycoanalytical procedures are required for the glycoscientific research field (e.g. metabolism of glycans, glyco-biomarker discovery, and quality control of glycoprotein pharmaceuticals). Among currently available analytical techniques, microchip electrophoresis is one candidate for high-throughput quantitative and qualitative glycoanalysis. In this article, we describe a strategy for high-throughput glycoanalytical procedures for the relative quantitation of N-linked glycans using the MultiNA microchip electrophoresis system, which is specialized for DNA/RNA analysis. We designed suitable derivatization of glycans for fluorescence detection in MultiNA and optimized separation conditions for derivatized glycans without any hardware modification. APTS-labeled N-glycans released from some glycoproteins were successfully separated based on the difference in branching type and terminal modifications within 1.5 min. Furthermore, we also designed microchip lectin-affinity electrophoresis for glyco-profiling. Thus, we showed that microchip electrophoresis provides high-throughput quantitative and qualitative glyco-analytical procedures and expect that MultiNA will provide a new dimension to glycoscience in the field of glycobiology.
Keywords: Microchip electrophoresis, Glycoprotein, N-glycans, Glycoanalysis
1Faculty of Pharmacy, Kindai University, Osaka, Japan
2Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
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