Plant Metabolome Analysis Method Using Mass Spectrometry and Its Application
by Junichi Masuda1, Mami Okamoto, Ph.D.2, Junko Takanobu2, Muneo Sato2, Yutaka Yamada2, Yuka Fujito3, Satoshi Yamaki, Ph.D.4, Yoshihiro Hayakawa3, Yuji Sawada, Ph.D.2, Masami Y. Hirai, Ph.D.2, and Masaru Furuta3
(Received December 27, 2017)
Metabolomic analysis using mass spectrometry is being widely used in various fields for the analysis of primary and secondary metabolites. In the field of plants, expectations have been growing for the application of metabolomic analysis to the evaluation of improved and high added-value plants. Here, we report on the results of an investigation into a plant metabolome platform using the breeding of Chrysanthemum (Chrysanthemum morifolium) as model plants. Metabolomic analyses of Chrysanthemum were performed using a gas chromatograph mass spectrometer (GCMS TQ-8040) and a Smart Metabolites Database package as well as a liquid chromatograph mass spectrometer (LCMS-8050). In the GC/MS analyses, we drew up a metabolites network map, which can assist in evaluations for breeding or quality control such as at harvest time, in addition to adding QC correction for improving reproducibility. We also developed a simultaneous analytical method for 17 major carotenoids that may play key roles in determining flower color. The results indicate that using the newly developed method the prediction of metabolite pathways is possible.
Keywords: Plant metabolome, Metabolomics, Mass spectrometry, APCI-MS, Primary metabolites, Secondary metabolites, Carotenoids, Chrysanthemum flower
1Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kanagawa, Japan
2RIKEN Center for Sustainable Resource Science, Kanagawa, Japan
3Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
4Shimadzu (China) Co., Ltd., Beijing, China
*The information contained in Shimadzu Review has not been modified since the original publication date. Please be aware that in some cases, products mentioned within the articles are no longer available.