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Development of Novel Tandem Mass Spectrometer (HAD-MS/MS) Using Gas-Phase Radicals
by Hidenori Takahashi, Ph.D.1
(Received January 15, 2019)
Tandem mass spectrometry (MS/MS) is an effective analytical technique for the characterization of structurally diverse biopolymers. However, conventional MS/MS, using collision-induced dissociation (CID) often provides insufficient product ions for the structural determination of unknown compounds, due to its lower reproducibility and specificity. To overcome this limitation, our research group developed a novel MS/MS technique using hydrogen attachment/abstraction dissociation (HAD). In this article, we introduce the usefulness of HAD for peptide sequence analysis with post-translational modification, double-bond position analysis of lipids, and the characterization of trans unsaturated fatty acids.
Keywords: Hydrogen, Lipid, Post-translational modification, Double-bond isomer, Trans unsaturated fatty acids
1Koichi Tanaka Mass Spectrometry Research Laboratory, Shimadzu Corporation, Kyoto, Japan
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