Shimadzu Review Vol.75 [3・4] (2018) | Development of Advanced Core Technology

SPECIALLY COLLECTED PAPERS

Multispectral Imaging of Fluorescent Micro Beads Using Multispectral Incoherent Holography (MIHO)

by Yuya Nagata1, Masaki Obara, Ph.D.1, Masahiro Ihara1, and Kyu Yoshimori, Ph.D.2
(Received December 27, 2018)

Abstract

We are developing a new technique of incoherent holographic spectrometry. It is a combination of Fourier transform spectroscopy (FTS) and incoherent digital holography, and it enables spectral information and 3D information to be obtained simultaneously by single scan. We call this technique multispectral incoherent holography (MIHO). We constructed a microscopic system and measured a point source to confirm the principle of MIHO, and demonstrated multispectral imaging of fluorescent beads. MIHO can obtain 3D spectral imaging easily because it does not require an XY scan and focusing as in a confocal laser microscopic system. We expect that MIHO will make a great contribution to medical research, such as new drug development.

Keywords: Incoherent holography, 3D imaging, Fourier transform spectroscopy, Multispectral imaging, Fluorescent imaging, Fluorescent microscopy

1Optical Technology Unit, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan

2Department of System Innovation Engineering, Faculty of Science and Engineering, Iwate University, Iwate , Japan

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