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－ Supporting Clinical Research from Testing to Therapeutic Agent Development －
Shimadzu Review 77[1・2] (2020.6)
PCR testing is used for rapid verification of infection with SARS-CoV2. One of the factors preventing increased use of PCR testing in Japan has been that many of the PCR tests currently in use require a complicated RNA purification step. To resolve this issue, we developed the 2019 Novel Coronavirus Detection Kit that requires no RNA purification. Also, to support clinical research into remdesivir, a drug that has received special approval as a treatment for COVID-19, we developed a simultaneous analysis method for remdesivir and its metabolite (GS-441524) that reduces the risk of specimen exposure by using an LC/MS/MS system with a fully automated sample preparation unit. As technology that is relevant to measures against SARS-CoV2 infection, this article describes the principle of the 2019 Novel Coronavirus Detection Kit and an evaluation of this kit using clinical specimens. This article also describes a simple and safe assay method for remdesivir and its metabolite using LC/MS/MS and a fully automated sample preparation unit.
1Clinical & Biotechnology Business Unit, Life Science Business Department, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
2Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
*The information contained in Shimadzu Review has not been modified since the original publication date. Please be aware that in some cases, products mentioned within the articles are no longer available.