Shimadzu Review Vol.77[1・2](2020)
Life Science


Development of a Method of Evaluating the Undifferentiated State of Pluripotent Stem Cells by Multicomponent Analysis of Culture Supernatant

by Kenichi Toyoda1Hirotaka Kuroda1Takako Yamamoto2Mao Arita2Takashi Suzuki, Ph.D.1Masatoshi Takahashi3Toru Ezure, Ph.D.1Shin Kawamata2

Shimadzu Review 77[1・2] (2020.7)


In working to develop noninvasive cell culture monitoring technology based on culture supernatant analysis, the authors previously developed a liquid chromatograph mass spectrometer-based method for the simultaneous analysis of culture components and secreted metabolites and used this method to identify kynurenine, a candidate marker characteris-tic for the undifferentiated state of pluripotent stem cells. The authors developed a new and more comprehensive method for simultaneous multicomponent analysis of culture supernatant by expanding the range of components measured by this method with a focus on secreted metabolites. The authors used this method to analyze the culture supernatant of undiffer-entiated cells and differentiated cells and identified N-formyl kynurenine, a new candidate marker characteristic of undif-ferentiated cells. N-formyl kynurenine demonstrates a marked change with cell differentiation at an earlier stage of culture than the above-mentioned kynurenine. This study shows that more effective marker candidates can be found by expanding the range of measured components. 

1Cell Business Development Section, Life Science Business Department, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
2Research & Development Center for Cell Therapy, Foundation for Biomedical Research and Innovation at Kobe, Kobe, Japan
3 Business Development Department, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan

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