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Shimadzu Review 77[1・2] (2020.7)
In working to develop noninvasive cell culture monitoring technology based on culture supernatant analysis, the authors previously developed a liquid chromatograph mass spectrometer-based method for the simultaneous analysis of culture components and secreted metabolites and used this method to identify kynurenine, a candidate marker characteris-tic for the undifferentiated state of pluripotent stem cells. The authors developed a new and more comprehensive method for simultaneous multicomponent analysis of culture supernatant by expanding the range of components measured by this method with a focus on secreted metabolites. The authors used this method to analyze the culture supernatant of undiffer-entiated cells and differentiated cells and identified N-formyl kynurenine, a new candidate marker characteristic of undif-ferentiated cells. N-formyl kynurenine demonstrates a marked change with cell differentiation at an earlier stage of culture than the above-mentioned kynurenine. This study shows that more effective marker candidates can be found by expanding the range of measured components.
1Cell Business Development Section, Life Science Business Department, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan
2Research & Development Center for Cell Therapy, Foundation for Biomedical Research and Innovation at Kobe, Kobe, Japan
3 Business Development Department, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan
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