Shimadzu Review Vol.77[3・4](2020)
Food Safety and Food Health


Development of an Ultra-Fast MRM-Based Analytical Method for Human Blood
Lipids and Applications in Food Analysis

by Masaki Yamada, Ph.D.1

Shimadzu Review 77[3・4] (2021.3)


The recent emergence of an ultra-fast multiple reaction monitoring (MRM) mode for triple-quadrupole mass spectrom-eters has enabled the development of analytical methods capable of analyzing hundreds of lipid molecules in biological samples, and these methods are being used to search for disease-related biomarkers. This article describes the development of an ultra-fast MRM-based analytical method for lipids in human serum and applications for this analytical method. The authors developed MRM transitions for lyso-phospholipids and diacyl-phospholipids detected in human serum by the phospholipid profiling method they already developed. Next, MRM transitions were added for triacylglycerols, cholesteryl-esters, cholesterol, and free fatty acids. Chromatography conditions were then improved and an analytical method was developed with a 19-minute analysis cycle. This analytical method detected over 100 major lipid components in human serum and differentiated between isomers of fatty acid combinations for target phospholipids and triacylglycerols. The analytical method was used to analyze lipids extracted from mackerel and discuss differences in the fatty acid balance of human serum and mackerel.

1Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan

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