Shimadzu Review Vol.78[1・2](2021)
Industrial Machinery


Development of an Analytical Method for Blood Triglycerides Using Triple Quadrupole Mass Spectrometer

by Yutaka Umakoshi1Toinon Doriane1Masaki Yamada, Ph.D.1

Shimadzu Review 78[1・2] (2021)


Triglycerides (TG) are one of the major types of lipid present in blood and essential to life due to the role they play as molecules that store and transport energy. Conventional blood tests estimate the total amount of triglycerides and provide no quantitative information about the various fatty acids (FAs) constituting triglycerides. This article describes the development of a method for quantitative analysis of fatty acids constituting triglycerides in blood using a high-performance liquid chromatograph-triple quadrupole mass spectrometer. The method uses 195 multiple reaction monitoring (MRM) transitions to allow the measurement of 47 triglycerides of different molecular weights in 11 minutes (130 analyses/day). This article also describes using this method to analyze two commercially available human plasma samples and one human serum sample. Triglyceride components detected in these samples underwent multivariate analysis to determine which accounted for slight differences between the samples. The different fatty acid balance of each sample was also visualized by showing each fatty acid on a radar chart.

1Global Application Development Center, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan

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