Shimadzu Review Vol.78[3・4](2021)
Development of Advanced Core Technology


Development of HYDROXTM: A Novel 3D Cell Culture Substrate Applications
in Induced Differentiation of iPS Cells into Hepatocytes and
Culture of Primary Human Hepatocytes

by Junko Enomoto, Ph.D. Hayato Matsui, Ph.D.

Shimadzu Review 78[3・4] (2021)


The field of regenerative medicine has attracted considerable interest in recent years and requires technology that can enable the easy production of highly functional three-dimensional cell aggregates. Extracellular matrices that contain animal-derived components such as Matrigel™ and collagen are the gold standard when it comes to substrates used in two-dimensional and three-dimensional cell cultures. However, problems have been noted with these extracellular matrices, such as their preparation from animals that gives rise to inconstant experimental results due to lot-to-lot differences between the animals, and because these matrices must be used at around 4 °C. Therefore, we developed a novel cell culture substrate (HYDROX™) from a chemically synthesized amphiphilic polymer containing hydrophilic poly(sarcosine) and hydrophobic poly(L-lactic acid). Cell culture on a synthetic chemical substrate promises to eliminate variability in experimental results and improve handling and safety. This article describes how HYDROX-coated plates are prepared for three-dimensional cell culture and the results from actual cell culture experiments performed on these plates.

Bio-industry Unit, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan

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