Shimadzu Review Vol.81[3・4](2024)
Realizing Health for People and the Planet through Food and Sustainable Food and Agriculture Industries

SPECIALLY COLLECTED PAPERS

Development of the FS FinderTM Series of Foodborne Pathogens Detection Kits with AmpdirectTM Technology
-Investigation of Direct Testing Methods from Food Culture Media-

by Hayato Koizumi, Ph.D.1Teppei Yazaki2Wakana Asada2Naoya Sakuragi, Ph.D.1Yu Arima1Nao Kondo, Ph.D.1Yusuke Shibahara, Ph.D.1Ayaka Nakamura, Ph.D.2Hajime Takahashi, Ph.D.2Toshihiro Ii1Yuichi Oku, Ph.D.1

Shimadzu Review 81[3・4] (2024)

Abstract

Food producers typically used culture-based methods to test for foodborne pathogens in food, then a growing demand for shorter testing times led to the development of PCR-based tests. However, the PCR-based tests available today still have major issues, such as the need for complex sample processing and high costs. We used Shimadzuʼs Ampdirect technology to develop real-time PCR assays (the FS Finder series) that can detect foodborne pathogens with minimal sample processing. This article describes a Salmonella spp. detection kit developed with primers and probes already in use in existing kits and published in past reports, and the Vibrio parahaemolyticus detection kit that is currently in development.

1Shimadzu Diagnostics Corporation, Tokyo, Japan
2Tokyo University of Marine Science and Technology, Tokyo, Japan

Request for Article

*The information contained in Shimadzu Review has not been modified since the original publication date. Please be aware that in some cases, products mentioned within the articles are no longer available.