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Using a Novel Ion Dissociation Method: Oxygen Attachment Dissociation (OAD)
Shimadzu Review 82[1・2] (2025)
Abstract
Tandem mass spectrometry (MS/MS) is an effective analytical technique for the characterization of structurally diverse biopolymers. The most common fragmentation technique used in MS/MS is collision-induced dissociation (CID). However, CID tends to fragment ions at locations with weak bond energies and often fails to provide sufficient structural information to characterize compounds with unknown chemical structures. To address this limitation, we have developed a novel mode of fragmentation called oxygen attachment dissociation (OAD) that is complementary to CID. OAD is a novel fragmentation technique developed by Shimadzu based on interactions between precursor ions and gas-phase radicals. It enables the characterization of molecular structures of biomolecules, including isomers, that are difficult to characterize by CID alone. This article presents an example application for OAD in untargeted lipidomics performed on an MS/MS system.
MS Business Unit, Life Science Business Department, Analytical Instruments Division, Shimadzu Corporation, Kyoto, Japan
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