Simultaneous Analysis of Monosaccharides and Oligosaccharides in Beer Using ELSD-LT III


User Benefits

- Simultaneous analysis of saccharides by gradient elution can be performed using ELSD-LT III evaporative light scattering detector. - Quantitative determination of Malto-oligosaccharides and isomalto-oligosaccharides in beer can be done owing to satisfactory separation of those saccharides each other. - Dynamic range expansion function enables quantitative determination at optimum sensitivity without manual consideration of sensitivity settings even if there are large differences in concentration of target compounds in beer.


Saccharide in beer is formed mainly through the saccharification of starch contained in malt and other raw materials, and one of the factors that determine the flavor of beer. Evaporative Light Scattering Detector (ELSD) is suitable for the detection of no-UV/VIS absorbing compounds such as saccharides. ELSD is also applicable to gradient elution, allowing simultaneous analysis of multiple compounds. In this Application News, we introduce a simultaneous analysis of monosaccharides and oligosaccharides contained in beer. Integrated high-performance liquid chromatograph i-Series LC-2050C with an ELSD-LT III was employed for this study. Hydrophilic interaction chromatography (HILIC), which is often used in saccharide analysis, enabled gradient elution using water and acetonitrile.

March 20, 2024 GMT

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