High Spatial Resolution Imaging by iMScope TRIO - Imaging of Chloroquine Distribution in Rat Retina -

Pharmacokinetic analysis of candidate compounds is a critical step in drug development, for elucidating the site and mechanism of activity and for evaluating local cytotoxicity.

Imaging mass spectrometry (MS) is a recently emerging technology as an alternative to conventional methods because it can quantify and give spatial distribution of both intact and metabolized forms of target compounds simultaneously. This label-free strategy is expected to drive drug development processes towards breakthrough discoveries. Here we present how imaging mass microscope iMScopeTRIOwas used for analysis of rat retina administered with chloroquine.
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High-Resolution Imaging of Chloroquine in Rat Retina

In this experiment, chloroquine, a well known drug against malaria infection, was administered to a rat to .elucidate its distribution in retina.
High resolution imaging of chloroquine distribution using a 10 μm section of pigment epithelium of retina was then performed by iMScopeTRIO. The MS/MS product ion spectrum of chloroquine (m/z320.190) and the plot of production intensity (m/z247.095) were displayed as .Fig. 1 and 2, respectively.

Table 1 Analytical Conditions

 Sample Type and Preparation
 Sample  Chloroquine treated (20 mg/kg, po) rat
retinal section (10 μm, OCT embedded)
 MALDI Matrix  CHCA applied by sublimation
 Analytical Conditions
 Polarity  positive mode
 Precursor Ion  m/z320.190
 Laser Diameter  10μm
 Acquisition  10μm


Fig. 1 MS/MS Product Ion Spectrum of Chloroquine Acquired Directly from Tissue Section

Fig. 2 MS/MS Imaging of Rat Retina, Showing the Spatial (Distribution of Chloroquine m/z 320.190 247.095)

Imaging Mass Solution

Imaging mass spectrometry is a revolutionary new technology.
The instrument is a combination of an optical microscope which allows the observation of high-resolution morphological images, with a mass spectrometer which identifies and visualizes the distribution of specific molecules.

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For Research Use Only. Not for use in diagnostic procedures.

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