Seed oil conjugated linolenic acid (CLnA) double bond characterization without chemical standards by solvent mediated chemical ionization tandem mass spectrometry

Polyunsaturated fatty acids with unusual double bond structure are found widely in nature. Their chemical characterization requires methods　that differentiate isomers of similar structures. Shimadzu’s solvent mediated chemical ionization unit for GC-MS/MS enables use of low volatility CI regents with specific properties. These capabilities are demonstrated here for the covalent-adduct CI methods applied to conjugated trienes in complex seed oil extracts, enabling double bond assignment without chemical standards.