Analysis of Metabolites in Serum Using GCMS/MS

Single quadrupole GC-MS provides excellent chromatographic resolution and enables stable measurements, and is therefore widely utilized for metabolome analyses involving the comprehensive analysis of in vivo metabolites. However, biological samples contain many metabolites and various matrices, so separation with single quadrupole GC-MS can be difficult. With triple quadrupole GC-MS/MS MRM, MS separation is performed twice, with Q1 and Q3. This helps remove the impact of overlapping peaks due to interfering components in comparison with scan mode, in which MS separation is performed with a single quadrupole, and thus enables the acquisition of accurate quantitative results with high-sensitivity detection.

This application data sheet presents an analysis of metabolites in standard human serum using the scan and MRM methods included in the GC/MS Metabolite Database Ver. 2, as well as a comparison of the results.