Quantitation of 33 benzodiazepines by LCMS-8030 from human serum using RECIPE ClinMass^® LC MS/MS Complete Kit MS6000

Benzodiazepines belong to a group of psychotropic drugs that are used for the treatment of anxiety and restlessness as well as for epileptic seizures. Diazepam, one of the most commonly prescribed benzodiazepines, is used to treat a number of conditions by the pharmacological action of enhancing the neurotransmitter GABA by binding to the GABA A receptor, causing CNS depression. Benzodiazepines comprise a heterocyclic unsaturated diazepine ring to which a benzene ring is attached. Most benzodiazepines also have a second six-membered ring with additional side chain groups which give different benzodiazepines their unique properties. Although considered safe, dose duration of 4 weeks is not normally exceeded because of the risk of dependence and other side effects. In situations of long-term administration the drug levels are monitored to optimize the administered drug dose. Due to the heterogeneity of the compound class the convenience of the pre-optimized kit accelerates method development by combining many compounds and respective internal standards which would be costly to source individually. In these experiments the RECIPE ClinMass^® LC MS/MS Complete Kit for Benzodiazepines in Serum was evaluated and optimized on the LCMS-8030.