Strategies for Multiple-Target Screening using LC-MS/MS with Merged Spectrum Database for Forensic Toxicology

In Forensic Toxicology, LC/MS/MS has become a preferred method for the routine quantitative and qualitative analysis of drugs of abuse. LC/MS/MS allows for the simultaneous analysis of multiple compounds in a single run, thus enabling a fast and high throughput analysis. In this study, we developed Multiple-Target Screening (MTS) method for forensic toxicology to reduce false positive and negative using MS/MS spectral library database. MTS method consists of multiple reaction monitoring (MRM) and product ion scans at three collision energies to confirm the compound identification based on mass spectral library searching. The mass spectral library was created using certified reference materials from over 1,200 compounds for forensic toxicology.